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. 2023 Mar 3;9(9):eadd0324. doi: 10.1126/sciadv.add0324

Fig. 3. HvCMF4 expression pattern and the coupled transcriptional programs.

Fig. 3.

(A) Relative expression (RT-qPCR) of HvCMF4 in different tissues. Data are shown as means ± SEM; n = 3 biological replicates; each replicate was the pool of 5 to 20 individuals. (B) ISH of HvCMF4 in developing spikes. Spike longitudinal (B1 to B3) or transverse sections (B4 to B6) are shown. B2 and B6 are zoom-in views from B1 and B5 dashed frames. Arrows indicate the junctional region of a spikelet and the main rachis, where spikelet-rachis intervascular connection is going to be established. Putative diffuse vascular bundles (left arrow) of the spikelet axis and enlarged vascular bundles (right arrow) of the rachis are indicated. A, anther; SE, sieve element; V, vasculature. (C) The coexpressed clusters identified on the basis of 4783 DYGs (see also fig. S10). Shown are three clusters with peak expression at W4.5 stage in rachis or spikelet. Gene number from each cluster is given below each cluster. (D) The enriched GO terms of genes from the three clusters in (C). (E) Chlorophyll autofluorescence imaging from a transverse section of BW spike at W4.5 stage. White dashed circle highlights cells where HvCMF4 is expressed. (F) ISH of HvGLK1 from longitudinal (F1) and transverse (F2) sections of BW spikes at W5.5 stage. Scale bars, 200 μm (B1, B3, and B5), 50 μm (B2, B4, and B6), 50 μm (E and F1 and F3), and 20 μm (F2).