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. 2023 Feb 9;12:e79511. doi: 10.7554/eLife.79511

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Gene (C. elegans) sams-1 Wormbase WBGene00008205
Gene (C. elegans) sams-4 Wormbase WBRNAi00010322
Gene (C. elegans) set-2 Wormbase WBGene00004782
Gene (C. elegans) set-16 Wormbase WBGene00011729
Gene (C. elegans) rbr-2 Wormbase WBGene00004319
Gene (C. elegans) spr-5 Wormbase WBRNAi00004611
Gene (C. elegans) lsd-1 Wormbase WBGene00011615
Genetic reagent (C. elegans) N2 Caenorhabditis Genetics Centre Wild type
Genetic reagent (C. elegans) sams-1(ok3033) CGC HA1975 sams-1(ok3033) X
Genetic reagent (C. elegans) sams-4(ok3315) CGC RB2420 C06E7.3(ok3315) IV
Genetic reagent (C. elegans) rfp::sams-1(ker5); sams-1(ok3033) This paper WAL500 WAL500. See ‘Materials and methods, section C. elegans strains’
Genetic reagent (C. elegans) gfp::sams-4(ker6); sams-4(ok3315) This paper WAL501 WAL501; See ‘Materials and methods, section C. elegans strains’
Genetic reagent (C. elegans) Pges-1::NHR-68::GFP Bulcha, J.T Cell Reports 26, 460–468.e4. 10.1016 /j.celrep.2018.12.064. VL1296 See ‘Materials and methods, section C. elegans strains’
Genetic reagent (C. elegans) sams-3::mKate(nu3139) This paper, InVivo Biosystems WAL503 WAL503; See ‘Materials and methods, section C. elegans strains’
Genetic reagent (C. elegans) rfp::sams-1;gfp(ker5)::sams-4(ker6) This paper WAL502 See ‘Materials and methods, section C. elegans strains’
Genetic reagent (C. elegans) Escherichia coli OP50 CGC N/A Used as food source for C. elegans
Strain, strain background (E. coli) sams-1 RNAi Source Bioscience X-5P21 Used to knock down target mRNA
Strain, strain background (E. coli) sams-4 RNAi Source Bioscience IV-3C01 Used to knock down target mRNA
Strain, strain background (E. coli) set-2 RNAi ORFeome RNAi library mv_C26E6.9 Used to knock down target mRNA
Strain, strain background (E. coli) set-16 RNAi Source Bioscience III-6D12 Used to knock down target mRNA
Strain, strain background (E. coli) rbr-2 RNAi Source Bioscience IV-5D22 Used to knock down target mRNA
Strain, strain background (E. coli) spr-5 RNAi Source Bioscience I-6H02 Used to knock down target mRNA
Strain, strain background (E. coli) lsd-1 RNAi Source Bioscience X-5P17 Used to knock down target mRNA
Antibody Tri-Methyl-Histone H3 Lys4 (Rabbit monoclonal) Cell Signaling Technology C42D8 Used for IF (1:200) and CUT&Tag (1:50)
Software, algorithm GraphPad Prism v8 https://www.graphpad.com N/A Used for statistical analysis of data and generate graphs
Software, algorithm HOMER https://homer.ucsd.edu/homer/, Heinz et al., 2010 Bioinformatic data analysis software
Software, algorithm Dolphin https://www.umassmed.edu/biocore/introducing-dolphin/, Yukselen et al., 2020 Bioinformatic data analysis software
Software, algorithm DeBrowser https://debrowser.umassmed.edu, Kucukural et al., 2019 Bioinformatic data analysis software
Software, algorithm ChipPeakAnno https://bioconductor.org/packages/release/bioc/html/ChIPpeakAnno.html, Zhu et al., 2010 Bioinformatic data analysis software
Software, algorithm BioVenn https://biovenn.nl, Hulsen et al., 2008 Used to generate Venn diagrams
Software, algorithm WormCat https://www.wormcat.com/, Higgins et al., 2022 Used to generate WormCat data
Software, algorithm BowTie2 https://bowtie-bio.sourceforge.net/bowtie2/index.shtml, Langmead et al., 2009 Bioinformatic data analysis software
Commercial assay or kit KAPA Library Quantification Kits https://sequencing.roche.com/global/en/products/group/kapa-library-quantification-kits.html#productInfo N/A Used to quantify DNA for CUT&Tag
Commercial assay or kit NEBNext High-Fidelity 2 X PCR Master Mix https://www.neb.com/products/m0541-nebnext-high-fidelity-2x-pcr-master-mix#Product%20Information N/A Used to amplify libraries in CUT&Tag
Chemical compound, drug ConA beads https://www.bangslabs.com/sites/default/files/imce/docs/PDS%20720%20Web.pdf N/A Used in CUT&Tag ‘See Materials and methods section; CUT&Tag’
Other Potter-Elvehjem Tissue Grinder With PTFE Pestle https://www.thomassci.com/Equipment/Grinders/_/POTTER-ELVEHJEM-TISSUE-GRINDER-WITH-PTFE-PESTLE?q=Kontes%20Glass 3432 N59; Mfr. No. 886000–0020 Used for homogenizing worms in CUT&Tag ‘Materials and methods section CUT&Tag’