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. 2023 Jan 25;12:e80594. doi: 10.7554/eLife.80594

Figure 5. The metamorphic transformation of selected mushroom body input neurons (MBINs) during early metamorphosis and their mature phenotype in the adult.

Confocal images track the GFP expression through the first 48 hr after pupariation (P); the adult images show flip-switch induced expression of red fluorescent protein. Arrowheads: growth cones; P+#: # hours after pupariation. Background staining for the developmental series is for Fasciclin II (magenta); for the adult, it is Bruchpilot (nc82) (blue). Lines used for developmental timelines: DAN-c1: JRC-SS03066, DAN-d1: JRC-MB328B, DAN-g1: JRC-SS01716, MBIN-l1: JRC-SS04484, MBIN-b1,-b2: JRC-SS21716.

Figure 5.

Figure 5—figure supplement 1. Confocal images following the degeneration of GFP-labeled larval PAM neuron early in metamorphosis.

Figure 5—figure supplement 1.

The cells are reduced to GFP-labeled fragments by 24 hr after pupariation. Inset is an enlarged version of the boxed region for line SS01949 showing the discrete axonal tufts in the shaft (SHA), upper toe (UT), and intermediate toe (IT) compartments. P+#: # hours after pupariation. Background staining for the developmental series is Fasciclin II (magenta); lines used for developmental timelines: DAN-h1, -i1, and -j1: JRC-SS01949; DAN-k1: JRC-SS01757.
Figure 5—figure supplement 2. Confocal images showing the early metamorphic changes in line JRC-SS01702 driving GFP expression in two mushroom body input neurons (MBINs): OAN-e1 and DAN-c1.

Figure 5—figure supplement 2.

The subsequent images track the axonal tufts of the two neurons. That of DAN-c1 persists as a cohesive tuft associated with the mushroom bodies while OAN-e1 tufts retract from the mushroom bodies form a diffuse arbor over the superior medial protocerebrum. The adult image shows an example of flip-switch-induced expression of red fluorescent protein (RFP) in the adult. Two adult neurons were found in flip-out samples of this line: the above cell interpreted as the adult version of OAN-e1 and the adult version of DAN-c1 as confirmed by a second, clean DAN-c1 line (Figure 5). P+#: # hours after pupariation. Background staining for the developmental series is for Fasciclin II (magenta); for the adult it is nc82 (blue).