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| Gene/RNA | Forward primer (5’→3’) | Reverse primer (5’→3’) |
|---|---|---|
| Genotyping | ||
| Cep78-null allele | CTCTGGTCATCCCTTTGTCTAATT | TAAGACCAAACGACTTCCTCAAAC |
| Wild type Cep78 allele | CTCTGGTCATCCCTTTGTCTAATT | CCTCTAAGAGCCTTACATAACTGG |
| Quantitative PCR (Q-PCR) | ||
| Cep78 | GTTGGTTCTGAAAACTGGAATAGG | GTCCATTGGCAAATATTAACTTGAA |
| Ift20 | TGAACTGAACAAGCTCCGAGT | GCAAGTTCCGAGCACCAAT |
| Ttc21a | GGCAGGATTGGGATCAGACG | GGTTTGGATTGTGGGGCTCT |
| 18S rRNA | CATTCGAACGTCTGCCCTATC | CCTGCTGCCTTCCTTGGA |
