| Gene (Homo sapiens) |
P65 |
hg38 |
ENSG00000173039 |
|
| Gene (Homo sapiens) |
PODXL |
hg38 |
ENSG00000128567 |
|
| Gene (Homo sapiens) |
ATP1A1 |
hg38 |
ENSG00000163399 |
|
| Gene (Homo sapiens, Mus musculus) |
RPL7 |
hg38, mm10 |
ENSG00000147604, ENSMUSG00000043716 |
|
| Gene (Homo sapiens, Mus musculus) |
RPS28 |
hg38, mm10 |
ENSG00000233927, ENSMUSG00000067288 |
|
| Gene (Homo sapiens, Mus musculus) |
LARP1 |
hg38, mm10 |
ENSG00000155506, ENSMUSG00000037331 |
|
| Gene (Homo sapiens, Mus musculus) |
NET1 |
hg38, mm10 |
ENSG00000173848, ENSMUSG00000021215 |
|
| Sequence-based reagent |
LARP1 guide RNA exon2 |
Synthego |
GCUGUUCCUAAACAGCGCAA |
Used to create human LARP1-null cells |
| Sequence-based reagent |
LARP1 guide RNA exon19 |
Synthego |
CAACCCCCUACACCACCCAC |
Used to create human LARP1-null cells |
| Sequence-based reagent |
AAVS1 guide RNA |
Synthego |
UAGUGGCCCCACUGUGGGGU |
Used to create human loxP cells |
| Sequence-based reagent |
Larp1 siRNA |
IDT |
mm.RI.Larp1.13.2 |
Used as equimolar mix |
| Sequence-based reagent |
Larp1 siRNA |
IDT |
mm.RI.Larp1.13.3 |
Used as equimolar mix |
| Sequence-based reagent |
SSB siRNA |
IDT |
mm.RI.Larp1.13.1 |
Used alone |
| Sequence-based reagent |
Larp4 siRNA |
IDT |
mm.RI.Larp1.13.1 |
Used alone |
| Sequence-based reagent |
LARP7 siRNA |
IDT |
mm.RI.Larp1.13.1 |
Used alone |
| Sequence-based reagent |
FLAP_Y |
IDT |
/5Cy3/AA TGC ATG TCG ACG AGG TCC GAG TGT AA/3Cy3Sp/ |
hybridized to smiFISH probes |
| Sequence-based reagent |
RPL7 5'TOP |
IDT |
CCTCTTTTTCCGGCTGGAACC |
used for cloning into reporter constructs |
| Sequence-based reagent |
RPL7 mutant 5'TOP |
IDT |
AAGAGGGGGAAGGAGGGAAAA |
used for cloning into reporter constructs |
| Sequence-based reagent |
RPS28 5'TOP |
IDT |
ACTCCTCTCCGCCAGACCGCCGCCGCGCCGCCATC
|
used for cloning into reporter constructs |
| Sequence-based reagent |
RPS28 mutant 5'TOP |
IDT |
AAGAAGAGAAGAAAGAAAGAAGAAGAGAAGAAAGA
|
used for cloning into reporter constructs |
| Cell line (Mus musculus) |
CAD |
Sigma |
08100805-1VL, RRID:
CVCL_0199
|
|
| Cell line (Mus musculus) |
CAD/loxP |
Khandelia et al., 2011
|
|
Contains single integration of loxP cassette |
| Cell line (Homo sapiens) |
C2bbe1 |
ATCC |
CRL-2102 |
|
| Cell line (Homo sapiens) |
C2bbe1/loxP |
Khandelia et al., 2011
|
|
Contains single integration of loxP cassette |
| Cell line (Homo sapiens) |
HCA-7 |
ECACC |
6061902 |
|
| Cell line (Homo sapiens) |
HCA-7/loxP |
Khandelia et al., 2011
|
|
Contains single integration of loxP cassette |
| Cell line (Canis lupus) |
MDCK |
ATCC |
CRL-2935 |
|
| Cell line (Canis lupus) |
MDCK/loxP |
Khandelia et al., 2011
|
|
Contains random integration of loxP cassette |
| Transfected construct (Homo sapiens) |
PODXL cDNA |
Horizon |
MHS6278-202858197 |
used for cloning into Halo plasmids |
| Transfected construct (Homo sapiens) |
ATP1A1 cDNA |
Horizon |
MHS6278-202759485 |
used for cloning into Halo plasmids |
| Transfected construct (Homo sapiens) |
P65 cDNA |
R.Spitale, UC Irvine |
|
used for cloning into Halo plasmids |
| Transfected construct (Homo sapiens) |
LARP1 cDNA |
Horizon |
MHS6278-202827213 |
used for cloning into Halo plasmids |
| Antibody |
mouse monoclonal anti LARP1 |
Santa Cruz |
sc-515873 |
1:100 dilution for immunoblotting |
| Antibody |
mouse monoclonal anti ACTB |
Sigma |
A5441 |
1:5000 for immunoblotting |
| Antibody |
mouse monoclonal anti H3 |
Abcam |
10799 |
1:10000 for immunoblotting |
| Antibody |
rabbit polyclonal anti TOM20 |
ProteinTech |
11802–1-AP |
1:250 for immunofluorescence |
| Antibody |
rabbit polyclonal anti EZRIN |
Cell Signaling Technology |
3145 S |
1:250 for immunofluorescence |
| Antibody |
mouse monoclonal anti NaKATPase |
DSHB |
A5-s |
1:50 for immunofluorescence |
| Sequence-based reagent |
smFISH probes against Firefly luciferase |
BioSearch |
VSMF-1006–5 |
|
| Commercial assay or kit |
Zymo Quick-RNA Microprep kit |
Zymo Research |
R1050 |
|
| Commercial assay or kit |
Zymo Quick-RNA Miniprep kit |
Zymo Research |
R1055 |
|
| Commercial assay or kit |
Roche RNA HyperPrep Kit with HMR depletion |
Roche |
|
|
| Other |
Cell culture inserts for differentiation |
Corning |
353090 |
C2bbe1 are differentiation on 0.4 uM transwell inserts |
| Other |
Kinesore |
Sigma-Aldrich |
SML2361 |
Kinesin-1 Inhibitor |
| Other |
Ciliobrevin-A |
Selleckchem |
302803-72-1 |
Dynein Inhibitor |
| Other |
MitoView Green |
Biotium |
70,054 T |
100 ng/mL for immunofluorescence imaging of mitochondria |
| Other |
Halo-Dibromofluoroscein |
R.Spitale, UC Irvine |
|
ROS producing small molecule for Halo-seq labeling |
