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. 2022 Oct 5;30(3):673–686. doi: 10.1038/s41418-022-01071-3

Fig. 2. Osteoclast Hsp90ab1 deletion reduces osteoclastogenesis.

Fig. 2

A, B Relative mRNA expression of Hsp90ab1 and Hsp90aa1 in BMMs from Hsp90ab1f/f; LysM-Cre (f/f; Cre) mice or Hsp90ab1f/f (f/f) littermates. C Protein level of Hsp90β in BMMs. D TRAP staining of BMMs treated with 100 ng/ml RANKL and 30 ng/ml M-CSF for 7 days. Scale bars, 100 μm. E Quantification of TRAP-positive multinuclear cells. F BMMs were fixed and stained for F-actin with TRITC-phalloidin. Scale bars, 100 μm. G Number of osteoclasts with actin ring structures. H qRT-PCR was used to quantify relative mRNA expression levels of indicated genes. Bars represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Each experiment was performed at least three times.