Fig. 5. MAJIQ HET + VOILA Modulizer defines the complex landscape of cerebellar splicing changes.

a Pairwise comparisons run through MAJIQ HET to find significant splicing changes between GTEx cerebellar tissues (cerebellum and cerebellar hemisphere) versus the other 11 brain tissue groups. Dark arrows indicate an example of a consistent change, where both cerebellar tissue groups versus the same other brain region, the hypothalamus, shared a significant change. Alternative splicing (AS) modules were kept for downstream analysis if at least one such consistent comparison was significant (see “Methods”). GTEx abbreviations are given for each tissue. b Upset plot showing the consistent, significantly changing AS event type(s) that make up AS modules. AS events had to have an absolute difference in median $\mathbb{E}\left[\mathrm{\Psi }\right]$ of 20% or more when comparing both cerebellar tissue groups (cerebellum and cerebellar hemisphere) to the same other brain region tissue group in addition to having a Mann–Whitney two-sided p < 0.05 as reported by MAJIQ HET. c Examples of cassette exon (CE) types with consistent changes between cerebellar and other brain tissues. All CEs have quantified inclusion junctions (blue junctions) and a shared exclusion junction (red junction), potentially within a mixture of other AS event types (gray junctions and introns) (purple circles on upset plot in b).