Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Mar 3;14:1210. doi: 10.1038/s41467-023-36914-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a Percentage of PDGFRα⁺/PECAM1^- cells in mESC cultured with iCRT3. Mean ± SD; n = 3 independent experiments; two-tailed unpaired t test. b qRT-PCR of PE markers in mESCs cultured with iCRT3. Values represent Log₂ of fold change expression relative to DMSO. Log₂FC ± SD; n = 3 independent experiments; multiple unpaired t tests with Holm-Sidak method. c Representative flow cytometry plots of WT and Tcf7l1^−/− cells co-stained for PECAM1 and PDGFRα. d Percentage of PDGFRα⁺/PECAM1^- cells in WT and Tcf7l1^−/− mESCs. Mean ± SD; n = 3 biologically independent samples; two-tailed unpaired t test. e Representative flow cytometric plots of WT and Tcf7l1^−/− cells co-stained for PECAM1 and PDGFRα markers upon RA treatment. n = 3 independent experiments. f Flow cytometry analysis of PDGFRα and PECAM1 populations at 96H of RA treatment. Mean ± SD; n = 3 independent experiments; two-way ANOVA test. g qRT-PCR of PE markers in WT and Tcf7l1^−/− mESCs upon RA treatment. Mean ± SEM; n = 3; two-way ANOVA test. h Representative IF image of GATA6 and NANOG in WT and Tcf7l1^−/− mESCs upon RA treatment. Scale bar = 50 μm. i Quantification of NANOG⁺ and GATA6⁺ cells of Fig. 3h. Mean ± SD; two-tailed unpaired t test. Each dot represents % of cells in a field of view; WT:n = 12 non-overlapping images, Tcf7l1^−/−: n = 14 non-overlapping images from n = 1 experiment. j qRT-PCR of pluripotency markers in WT and Tcf7l1^−/− mESCs upon RA treatment. Mean ± SEM; n = 3 independent experiments; two-way ANOVA test. k qRT-PCR of embryonic neuroectodermal markers in WT and Tcf7l1^−/− mESCs upon RA treatment. Gene expression values are reported as Log₂ of fold change expression. n = 3 independent experiments. l (Left) Representative IF image of NESTIN in WT and Tcf7l1^−/− mESCs upon RA treatment. (Right) Quantification of NESTIN⁺ cells. Mean ± SD; two-tailed unpaired t test. Each dot represents % of cells in a field of view; WT: n = 12 non-overlapping images, Tcf7l1^−/−: n = 11 non-overlapping images from n = 1 experiment. Source data for all experiments are provided as a Source data file.