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. 2021 Oct 9;30(3):605–617. doi: 10.1038/s41418-021-00888-8

Fig. 2. The m6A level of RMRP was upregulated in NSCLC cells than lung epithelial cells.

Fig. 2

A The m6A methylation level of RMRP in human normal lung epithelial cells (HBE) and NSCLC cells (H1299 and A549) were determined by MeRIP-qPCR assays. The input RNA fraction Ct value was used to account for RNA sample preparation differences; negative control groups (IgG) were used to adjust background fraction (n = 3). B Results of Western blot assay showing METTL3 protein expression in A549 cells transfected with two different short interference RNAs for METTL3 (si-METTL3-1 and si-METTL3-2) or negative controls (si-Control) (n = 3). C Results of qRT-PCR assays showing the mRNA levels of METTL3 and RMRP in the treated A549 cells (n = 3). β-actin was used as the reference for normalization. D The m6A level in the treated A549 cells (n = 3). E Reduction of RMRP RNA stability in METTL3-knockdown A549 cells as compared to control. Cells were treated with 5 μg/mL actinomycin D and RNA was isolated at 0 h, 2 h, and 4 h (n = 3). Data presented as mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.