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. 2021 Oct 9;30(3):605–617. doi: 10.1038/s41418-021-00888-8

Fig. 3. RMRP promotes NSCLC cell proliferation.

Fig. 3

A Results of quantitative RT-PCR (qRT-PCR) analysis showing RMRP expression in NSCLC cell lines (H1299, H460, H1915, A549) and human lung epithelial cell line (HBE). β-actin was used as the endogenous control (n = 3). B Results of qRT-PCR analysis showing RMRP expression in A549 cells transfected with two different shRNAs against RMRP (shRMRP-1 and shRMRP-2) or scrambled control (Scrambled). β-actin was used as the endogenous control (n = 3). C Results of qRT-PCR analysis showing RMRP expression in H1299 cells transfected with RMRP-overexpressing plasmid (RMRP) or matched Controls (Ctrl). β-actin was used as the endogenous control (n = 3). D CCK-8 assays of A549 cells transfected with shRMRP compared with those transfected with Scrambled (n = 3). E CCK-8 assays of H1299 cells transfected with RMRP or Ctrl (n = 3). F Representative images (Up) and quantification (Down) of colony formation assays in A549 cells transfected with shRMRP or Scrambled (n = 3). G Representative images (Up) and quantification (Down) of colony formation assays in H1299 cells transfected with RMRP or Ctrl (n = 3). Data presented as mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.