REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
hiPSC-CMs	Ncardia	Pluricyte® Cardiomyocyte, PCMI-1033-1
hiPSC-CMs	Humanitas Clinical and Research Center-IRCCS	See “Experimental Model and subject details” section for the differentiation protocol.
Chemicals, peptides, and recombinant proteins
Fura-2-AM	Thermofisher Scientific	F1201
hiPSC growth medium	Thermofisher Scientific	StemFlex Medium (A3349401)
Vitronectin (VTN-N) Recombinant Human Protein, Truncated	Thermofisher Scientific	A14700
hiPSC-CMs medium	Ncardia	Pluricyte® Cardiomyocyte Medium, PM-2100-100mL
B27-Supplement w/o insulin	Thermofisher Scientific	0050129SA
B27-Supplement	Thermofisher Scientific	17504044
Growth Factor-Reduced Matrigel (10ml)	BD Biosciences	354230
RPMI Medium 1640 with L-Glutamine	Thermofisher Scientific	11875-119
CHIR-99021	Selleckchem	S1263-5MG
IWR1	Sigma Aldrich	10161-5MG
Y-27632 (ROCK Inhibitor)	Selleckchem	S1049
Laminin	Sigma Aldrich	L2020
Fibronectin	Sigma Aldrich	F0895-2MG
Software and algorithms
Microsoft Excel	Microsoft	https://www.microsoft.com/
GraphPad Prism	Prism	https://www.graphpad.com/
MATLAB	MathWorks	https://it.mathworks.com/
Origin 9.0	OriginLab Corporation	https://www.originlab.com/
pClamp-10 software	Axon Instruments	http://go.moleculardevices.com/
Contraction analysis code	This paper	[GitHub]: [https://github.com/BeatriceFederici/contraction_tracking.git]
Other
Patch-Clamp Amplifier	Molecular Devices	Axopatch 200B
Patch-Clamp Amplifier	Molecular Devices	MultiClamp 700B
Analog to digital converter	Molecular Devices	Digidata 1440A
Microscope	Nikon	Eclipse Ti
Microscope	Nikon	Eclipse TE200
Illumination source	Lumencor	Spectra X
Illumination source	Cairn Research	Arc Lamp Light Source coupled with high-speed OptoScan monochromator
Camera	Photometrics	Prime 95B Scientific CMOS (sCMOS) Camera
Camera	ThorLabs	CS135MUN, Kiralux 1.3 MP NIR-Enhanced CMOS Camera