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. 2023 Feb 3;26(3):106131. doi: 10.1016/j.isci.2023.106131

Figure 6.

Figure 6

PPT1 depalmitoylates HSP90α and abolishes DHT-induced AR activation

(A) Endogenous HSP90α palmitoylation levels after transfection with HA-tagged empty vector or HA-tagged PPT1 in GRM02 cells.

(B) Representative spectrum for the HSP90α peptide.

(C) GRM02 cells were transfected with HA-tagged PPT1 and then treated with DHT (100 nM) for 24h. Cell lysates were collected to determine the mRNA level of Cyp19a1. Cell supernatants were collected to determine the estradiol (D), testosterone (E) levels, the estrone (G) and androgen levels (H). (F) Estradiol to testosterone ratios; analysis based on the data from (D) and (E) (n = 12). For (D, E, and H), p values were determined by two-tailed Student’s t test, and data are presented as the mean value ± SEM. For (G), p values were determined by a two-tailed Mann–Whitney Wilcoxon test, and data are presented as the mean values ± SEM.