EA inhibited the up-regulated expression of P2Y1R in the prefrontal cortex and hippocampus from immunofluorescent staining. The immunofluorescent staining of P2Y1R and DAPI in the prefrontal cortex and hippocampus in the following five groups: GH, SI, EA, SEA, EA + 2365 groups. Scale bar = 20 μm. EA electroacupuncture, GH group housed, SI social isolated, SEA sham electroacupuncture