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. 2022 Oct 12;77(6):1968–1982. doi: 10.1002/hep.32776

FIGURE 3.

FIGURE 3

IL‐18 receptor deficiency significantly reduced expression of the HSC activation marker in 24‐week‐old mice with HSC‐specific NLRP3 hyperactivation. (A) mRNA levels of Ctgf, Col1a1, and Acta2 in control, Nlrp3 L351P/+ CreLrat Il18r +/+ , and Nlrp3 L351P/+ CreLrat Il18r −/− mice. (B) Representative pictures of H&E‐, Sirius Red‐, and F4/80‐stained liver sections. (C) Liver aminotransferases and quantification of (D) Sirius Red and (E) F4/80‐positive cells in mice with Il‐18R deficiency compared with Nlrp3 L351P/+ CreLrat Il18r +/+ and control mice. Acta2, actin alpha 2; Col1a1, collagen type I alpha 1; Ctgf, connective tissue growth factor; F4/80, murine macrophage marker; H&E, hematoxylin–eosin; HSC, hepatic stellate cell; Lrat, lecithin retinol acyltransferase; NLRP3, nucleotide‐binding oligomerization domain leucine rich repeat containing receptor‐family pyrin domain‐containing 3. n = 3–5 mice per group for all measured values; scale bars represent 100 μm. *p < 0.05; **p < 0.01.