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. 2023 Feb 11;28:366–384. doi: 10.1016/j.omtm.2023.02.004

Figure 7.

Figure 7

Nalm6 B cell line expression of eCD4-Ig-KiHR prevents NL4-3 HIV-1 spread in SupT1 CD4+ T cells in a cell co-culture model of HIV-1 infection

Nalm6 B cells were transduced with either M-eGFP-eCD4-Ig-KiHR or EB-eGFP-eCD4-Ig-KiHR LVs and later mixed at a 1:10 or a 1:4 ratio of Nalm6 eCD4-Ig-expressing cells to NL4-3 HIV-1-infected SupT1 CD4+ T cells. At the time of addition to Nalm6 B cells for co-culturing, SupT1 CD4+ T cells were ∼10% NL4-3 HIV-1 positive by p24 flow cytometry. Mock LV transduced Nalm6 cells (red lines) were used as controls. Cultures evaluated for HIV-1 p24 protein production (black solid lines, left axis) and eCD4-Ig production (dotted lines, right axis) on days 1, 3, and 6 post culture establishment. Dot plots indicate average protein production from four repeats, and error bars show SD. p < 0.001 for both co-culture ratios and for M and EB eCD4-Ig regulated LVs.