Figure 2.

Establishment of the microvasculature formation and development

(A+B) Schematic of the patterning of two parallel microvessels into fibrin hydrogel to study sprouting angiogenesis (A) and vasculogenesis (B).

(C) Exemplary z-projected fluorescence microscope images of two parallel microvasculatures and a close-up view of the region between the vessels. (blue: Hoechst, green: Pecam-1, red: F-Actin, Day: 8) (D) Exemplary z-projected fluorescence microscope images of two parallel microvasculatures surrounded by endothelial cells within the hydrogel matrix and a close-up view of the region between the vessels. (blue: Hoechst, green: Pecam-1, red: F-Actin, Day: 5).