Figure 2.

SIRT5 and lysine acetyltransferases regulate histone malonylation

(A) Western blot (WB) of acetylation, malonylation, succinylation, and glutarylation in histone extracts derived from wildtype and SIRT5 knockout (S5KO) mouse livers.

(B) Schematic of the regulation of histone malonylation. KMaT, lysine malonyl-transferase.

(C) Lysine acetyltransferase (KAT) knockdown K562 cell lines were constructed using a CRISPR-dCsa9-KRAB system via lentiviral infection. Knockdown efficiencies were examined by qPCR.

(D) WB examination of malonylation on histones extracted from KAT knockdown K562 cells treated with 25 μM orlistat for 24 h WT cells without lentiviral infection, or with scramble lentiviral infection (sgNC), as control cell lines (shown in blue).

(E) Quantification of the optical densities (OD) of malonylation blots normalized to Ponceau S staining.