Figure 3.
KAT2A regulates histone malonylation
(A) Western blot (WB) of malonylation in histone extracts. sgKAT2A (two different sgRNAs), sgNC, WT, and sgSIRT5 (two sgRNAs) K562 cell lines were treated with 25 μM orlistat or DMSO for 24 h.
(B) Optical density (OD) quantification of histone malonylation normalized to Ponceau S staining in (A).
(C) WB of malonylation in KAT2A single knockdown and SIRT5;KAT2A double knockdown K562 cells.
(D) OD quantification of histone malonylation normalized to Ponceau S staining. n = 4 per cell type. Error bar: mean ± SEM. ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 10−4 using unpaired Student’s t test.
(E) WB of malonylation in histone extracts. Empty vector or KAT2A O/E vector transfected into the corresponding K562 cells for 48 h. Cells were treated with 25 μM orlistat for the last 24 h.
(F) OD quantification of histone malonylation normalized to Ponceau S staining in (E).