Table 2.

Nucleotide sequences of the PCR primers used to evaluate mRNA abundance by RT-PCR (qPCR).

Gene	Forward	Reverse	Annealing temperature (ºC)	GeneBank acession number
acly	AGAGACGGCAGTAGCCAAAA	ATTGGCCACCAGTTCATCAT	56.0	*
actb	TCCTGCGGAATCCACGAGA	AACGTCGCACTTCATGATGCT	60.9	AY148350.1
cpt1a	TGCCAAGAGGTCATCCAGAGTTCT	AGTCCACATCATCCGCCAGAGA	64.2	KF857302
eef1a1	CGTTGGCTTCAACATCAAGA	GAAGTTGTCTGCTCCCTTGG	55.5	AJ866727
fasn	CGTCAAGCTCTCCATCCCTG	GGTGGTGTCTAGGCAGTGTC	59.6	MF566098
fbp1	GCTTTGACCCACTGGATGGT	GGCGGGATCAAGCATAAAGC	58.4	*
g6pc1	TGAGACCCGGTTTTATGGAG	CATGCAGACCACCAGCTCTA	57.0	AM987970
g6pd	GAGATGGTGCAGAACCTCATGG	CCACAGAAGACATCCAGGATGAG	62.0	JX073705
gck	ATCGTCAGGGAACTCACACC	GAGTTCAGGCTTGCTTCACC	58.3	AM986860
glud1	CCATCAGCCAGGGAGGAATC	TGCTCATGTAAGACGCCTCG	58.7	KF857576
got1	CTGGGTGGTACAGGTGCTTT	AGCGTTGTGATTTTCCCAAG	55.8	*
gpt	TGAAGGAGGGGGTCAAGAAA	AGGGTAAGAACACAGAGCCA	57.4	JX073702
gys2	GACAAGGAGGCAGGTGAGAG	GAAGACGTGAGCACAGTGGA	59.2	*
hadh	CGGAAGTGGTCAGATGGGTG	TCGTCGCTTGTATCCACCAG	59.0	KF857303
hk1	CACCAATGCGTGCTACATGG	GTCGACGTCCCTGTCAAACT	58.1	*
pck1	GCGCCATCAACACTAAAGGT	TTGTGCACTCTGTCCTCCAG	57.2	DV217087
pklr	CTGTTTCCTGTGGAGGCAGT	CAGCACAGCATTTGAAGGAG	55.8	AM981422
pygl	TTCCCAGACAAAGTCGCTGT	GTCTTGTGAGGTCCCATGC	58.2	*
18s	CCGCTTTGGTGACTCTAGATAACC	CAGAAAGTACCATCGAAAGTTGATAGG	59.0	AY831388.1

acly, ATP citrate lyase; actcb, β-actin; cpt1a, carnitine palmitoyltransferase 1a; eef1a1, elongation factor 1α; fasn, fatty acid synthase; fbp1, fructose-1,6-biphosphatase; g6pc1, glucose-6-phosphatase; g6pd, glucose-6-phophate-dehydrogenase; gck, glucokinase; glud1, glutamate dehydrogenase; got1, aspartate aminotransferase 1; gpt, alanine transaminase; gys2, glycogen synthase liver form; hadh, hydroxyacyl-Coenzyme A dehydrogenase; hk1, hexokinase; pck1, phosphoenolpyruvate carboxylase cytosolic form; pklr, pyruvate kinase; pygl, glycogen phosphorylase liver form; 18s, ribosomal RNA 18s. The asterisk (*) means that accession number is pending validation by the National Center for Biotechnology.