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Overview of TBaM-seq protocol. (A) Primers for target-enrichment, each containing a 30-nt common adapter and 20-nt gene specific homology region. (B) Following single-tube RT, cDNA libraries can be enriched for transcripts of interest via a second-strand synthesis reaction with gene-specific primers as shown in (A) and subsequently PCR-amplified from the common adapter. (C) The percentage of reads mapping to 82 targeted genes in libraries prepared with either BaM-seq or TBaM-seq.
