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. 2023 Feb 28;18:1063–1083. doi: 10.2147/IJN.S398446

Figure 8.

Figure 8

The effect of exosomal lncRNA LOC103691165 on BMSC osteogenesis in the bone fracture microenvironment. (a) qRT-PCR was used to examine the expression of LOC102555570, LOC103691165, and LOC100909675. The bar graph shows the increased expression of the above lncRNAs in the exosomes secreted by macrophages after being stimulated using hypoxia or CSF. ##p < 0.01, #p < 0.05. (b). Alizarin red staining of BMSCs, viewed under the microscope or with the naked eye. After receiving exosomes carrying the LOC103691165 overexpression plasmid, the Alizarin-red-stained area of BMSCs was enlarged. By contrast, the area of BMSCs stained with Alizarin red was reduced after treatment with exosomes carrying LOC103691165-targeting siRNA. (c and d) The absorbance value of BMSCs at 570 nm after Alizarin red staining. The bar graphs show the differences between each group. ##p < 0.01, #p < 0.05. (e) The effect of LOC103691165 on the expression of osteogenesis-related genes in BMSCs. The bar graphs show that the exosomes overexpressing LOC103691165 promoted the expression of BMP-2, RUNX2, OPN, and OC in BMSCs, while exosomes carrying the LOC103691165-targeting siRNA reduced the expression of the above genes in BMSCs. ##p < 0.01, #p < 0.05. Each experiment was repeated five times. Microscopy: 100× magnification and 200 μm scale.