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. 2023 Feb 28;18:1063–1083. doi: 10.2147/IJN.S398446

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© 2023 Wang et al.

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The source of macrophage-derived exosomal lncRNA LOC103691165 in the bone fracture microenvironment. (a) Immunofluorescence image of LOC103691165 expression obtained using in situ hybridization. CD86 was used to label M1 macrophages, and CD206 was used to label M2 macrophages (green fluorescence). The LOC103691165 probe was labeled using red fluorescence. The images show that both M1 and M2 macrophages expressed LOC103691165. (b and c) The number of cells expressing LOC103691165. Five fields of view (at 200× magnification) were randomly selected to calculate the number of cells. The bar graph shows no significant difference between the numbers of M1 and M2 macrophages expressing LOC103691165. Ns, not significant (p > 0.05). Microscopy: 200× magnification and 50 μm scale. The experiment was repeated five times.