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. 2023 Mar 4;14:1244. doi: 10.1038/s41467-023-36755-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic representation of the two-step chemically induced carcinogenesis experiments. b–e Tumor number (b), percentage of survival (c), tumor number/mouse (d), and tumor size (e) were analyzed during the two-step chemically induced carcinogenesis experiments in (i) p53WT;D2WT, (ii) p53KO^+/−;D2WT and iii) p53KO^+/−;D2KO mice (n = 12 mice/group). f D2 mRNA expression was measured by Real-Time PCR in SCC tumors from dorsal skin of (i) p53WT;D2WT, (ii) p53KO^+/−;D2WT and iii) p53KO^+/−;D2KO mice (n = 12 mice/group). g–i mRNA expression of epithelial (g) and mesenchymal (h) markers and their ratio (i) was measured by Real-Time PCR in SCC tumors from dorsal skin of (i) p53WT;D2WT, (ii) p53KO^+/−;D2WT and (iii) p53KO^+/−;D2KO mice. j, Western Blot analysis of epithelial (E-Cadherin) and mesenchymal (Vimentin, ZEB-1, Snail, Slug) proteins in SCC tumors from dorsal skin of (i) p53WT;D2WT, (ii) p53KO^+/−;D2WT and (iii) p53KO^+/−;D2KO mice (n = 12 mice/group). A representative result of 3 independent experiments is shown. Tubulin was used as internal markers. k H&E of skin lesions from (i) p53KO^+/−;D2WT and (ii) p53KO^+/−;D2KO mice. Full skin-section reconstruction with Leica LAS X Navigator and representative zoom images. Magnification 20X. Scale bars represent 50 μm (representative of 3 images per sample, n = 3 mice). l IF staining with epithelial (E-Cadherin, Krt14) and mesenchymal (Vimentin, Krt8) markers of skin lesions from (i) p53KO^+/−;D2WT and (ii) p53KO^+/−;D2KO mice (representative of 3 images per sample, n = 3 mice). Magnification 20X. Scale bars represent 50 μm. All the results are shown as means ± SD from at least 3 separate experiments. p-values were determined by two-tailed Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.