FIG. 1.

(A) Schematic of the E2F chromatin immunoprecipitation cloning procedure. (B) Graphical representation of the results of a chromatin immunoprecipitation experiment measuring E2F binding at the Myc promoter, Myc exon 2, and Hox3D exon 2. Scores representing homology to the E2F consensus site as determined by computer analysis (21) are shown at the top of each graph. The y axis represents Imagequant quantitation of the amount of specific PCR products expressed as the percentage of antibody binding versus the amount of PCR product obtained using a standarized aliquot of input chromatin. The signal in the no-antibody lane was subtracted from each sample as a nonspecific binding background. The E2F family members used in the immunoprecipitation are shown on the x axis.