FIG. 2.

Confirmation of ChET clones by examining E2F binding in vivo. A representative chromatin immunoprecipitation experiment with HeLa cells is shown. Immunoprecipitation proceeded utilizing antibodies (Ab) against E2F1 (lane 1), E2F2 (lane 2), E2F3 (lane 3), E2F4 (lane 4), E2F5 (lane 5), and E2F6 (lane 6) or no antibody (lane 8). Following DNA purification, samples were subjected to PCR with primers designed for the individual E2F clones or the dhfr promoter as a control (labeled on the right). A portion of the total input was also examined by PCR (lane 7).