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Characterization of the protein complexes bound in vivo to the ChET clones. A chromatin immunoprecipitation experiment was performed in HeLa cells utilizing antibodies (Ab) to E2F1 (lane 1), E2F4 (lane 2), p107 (lane 3), p130 (lane 4), Rb (lane 5), RNA polymerase II (Pol II; lane 6), or no antibody as a control (lane 8). An aliquot of the total input is also shown (lane 7). Primers to the ChET clones or the DHFR promoter were used in PCRs for analysis.
