FIG. 7.

mRNA expression profiles of the three high-affinity ChET clones. (A) RT-PCR analysis of mRNA expression levels in RNA obtained from either U937 log-phase (lane 1) or differentiated (diff; lane 2) cells. RT-PCR primers complementary to E2F1, ChET 9, ChET 8, ChET 4, RNA polymerase (RNAP) II, or XRCC2 were used as indicated on the right. A water control is shown in lane 3. (B) RT-PCR analysis of RNA from either normal (N) colon (lane 1) or colon tumor (T; lane 2). Primer sets to the specific mRNAs are indicated. (C) RT-PCR analysis of ChET 9 mRNA expression in the RNA obtained from either human normal colon (lane 1), colon tumor (lane 2), normal liver (lane 3), or liver tumor (lane 4). The normal colon and colon tumor samples are the same as those shown in panel B with GAPDH primers added as a loading control.