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Effect of freezing/thawing cycle in gene editing and viability. (A) BCL11A editing efficiency comparison of freshly edited HSPCs versus thawed edited HSPCs after freezing storage at −80°C for 6 months, n = 1. (B) Time course analysis of BCL11A editing efficiency and cellular viability after thawing for large-scale Prodigy sample. (C) Time course analysis of cell number after thawing for large-scale Prodigy sample.
