FIGURE 3.

Differentiation of macrophages into M2‐like phenotype. A, B, Treatment with A549 or RERF‐LC‐MS‐conditioned medium increased the expression of CD206 on PMA‐treated THP‐1 cells. When A549 or RERF‐LC‐MS cells were cultured with nimesulide, the conditioned medium did not increase CD206 expression. Furthermore, recombinant MCP‐1 and PGE2 treatment recovered CD206 expression. C, Similarly, LLC‐conditioned medium increased the expression of CD206 on J774‐1 cells. When LLC cells were cultured with nimesulide, the conditioned medium did not increase CD206 expression. Furthermore, recombinant MCP‐1 and PGE2 treatment recovered CD206 expression. rMCP‐1, recombinant monocyte chemoattractant protein‐1; PGE2, prostaglandin E2