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SEC elution profiles for LgBiT-containing EVs
S15-LgBiT EV (+/- hACE2) - containing supernatants were harvested, concentrated, and purified by SEC. Fractions of eluate were collected in PBS. 10 μL of each 0.5-mL fraction were complemented with HiBiT in presence of nanoluciferase substrate and passive lysis buffer. The signals at fractions 7–9 include peak EV eluates. Error bars represent standard error of the mean (SEM).
