Reagent	Final concentration	Amount
Corning™ DMEM with L-Glutamine, 4.5 g/L Glucose and Sodium Pyruvate	N/A	480 mL
HEPES buffer (1 M)	10 mM	5 mL
Penicillin-Streptomycin solution (100×)	1×	5 mL
L-Glutamine (200 mM)	2 mM	5 mL
MEM Non-Essential Amino Acids Solution (100×)	1×	5 mL
Total	N/A	500 mL