REAGENT or RESOURCE	SOURCE	IDENTIFIER
Biological samples
COVID-19 convalescent sera samples	Dr. Susan Uprichard (Loyola University Chicago)	N/A
Chemicals, peptides, and recombinant proteins
DrkBiT peptide ≥90% purity (VSGWALFKKIS)	Genscript (Fast Peptide Synthesis)	N/A
HiBiT control protein	Promega	Cat#N3010
LgBiT protein (included in Nano-Glo HiBiT Extracellular Detection System kit)	Promega	Cat#N401A
Passive Lysis 5× Buffer	Promega	Cat#E1941
Trypsin from bovine pancreas, TPCK treated	Sigma-Aldrich	Cat#T1426-50MG
LipoD293	SignaGen	Cat#SL100668
Corning® 10× Phosphate-Buffered Saline (PBS), pH 7.4 ± 0.1	Fisher Scientific	Cat#46-013-CM
Corning™ DMEM with L-Glutamine, 4.5 g/L Glucose and Sodium Pyruvate	Fisher Scientific	Cat#MT10013CV
Penicillin-Streptomycin solution	Fisher Scientific	Cat#SV30010
Corning™ HEPES, Liquid	Fisher Scientific	Cat#MT25060CI
MEM Non-Essential Amino Acids Solution (100×)	Fisher Scientific	Cat#11-140-050
Cytiva L-Glutamine	Fisher Scientific	Cat#SH30034.01
Fetal bovine serum - premium, heat inactivated	R&D Systems	Cat#S11150H
Critical commercial assays
Nano-Glo HiBiT Extracellular Detection System	Promega	Cat#N2420
Nano-Glo Luciferase Assay System	Promega	Cat#N1110
Experimental models: Cell lines
HEK-293T	Laboratory of Edward Campbell (Loyola University Chicago)	N/A
Recombinant DNA
pcDNA3.1-SARS2-S-D614G	(Qing et al.1)	N/A
pcDNA3.1-SARS2-E	(Kumar et al.4)	N/A
pcDNA3.1-SARS2-M	(Kumar et al.4)	N/A
pcDNA3.1-SARS2- HiBiT-N	(Kumar et al.4)	N/A
pcDNA3.1- empty vector	Invitrogen	Cat#V79020
pcDNA3.1-hACE2-C9	Dr. Michael Farzan (Scripps Research Institute)	N/A
pcDNA3.1-S15-LgBiT	(Marcink et al.3)	N/A
pcDNA3.1-ACE2-LgBiT	(Kumar et al.4)	N/A
pCMV6-Entry-huDPP4 (MERS receptor)	Origene (Barlan et al.7)	N/A
pcDNA3.1- CEACAM-Long (MHV receptor)	(Boscarino et al.8)	N/A
pCAGGS-MHV-A59 S	(Boscarino et al.8)	N/A
pCAGGS-MHV-A59 E	(Boscarino et al.8)	N/A
pCAGGS-MHV-A59 HiBiT-N	(Kumar et al.4)	N/A
pCAGGS-MHV-A59 M	(Boscarino et al.8)	N/A
pcDNA3.1- MERS-EMC S-C9	(Barlan et al.7)	N/A
pcDNA3.1- MERS-EMC E	(Qing et al., 20209)	N/A
pcDNA3.1- MERS-EMC HiBiT-N	(Kumar et al.4)	N/A
pcDNA3.1- MERS-EMC M	(Qing et al., 20209)	N/A
Software and algorithms
Prism	GraphPad	N/A
Other
qEVoriginal / 35nm Legacy Column (SEC columns)	Izon	Cat#SP5
GloMax® Explorer Multimode Microplate Reader	Promega	Cat#GM3510
Corning™ 96-Well Solid White Polystyrene Microplates	Fisher Scientific	Cat#07-200-589
Amicon® Ultra-15 Centrifugal Filter Unit (100 kDa cutoff)	Millipore	Cat#UFC910024
Automatic fraction collector (AFC, V1)	IZON	N/A