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. 2023 Mar 6;9:81. doi: 10.1038/s41420-023-01383-4

Fig. 1. In vitro effect of RSL3+FAC treatment on PCa cells.

Fig. 1

A Western blot analysis of transferrin receptor (TfR-1) and Ferritin (Ft) in DU145 and TRAMP-C2 cells treated for 24 h with 100 μM FAC, 0.5 μM RSL3 or RSL3 + FAC. Tubulin was used for normalization. Viable cell count of DU145 and TRAMP-C2 cells treated for 48 h with increasing concentrations of FAC (25–500 μM) (B), RSL3 (0.0001–10 μM) or RSL3 + FAC (0.0001–10 μM RSL3 + 100 μM FAC) (C). D Motility assay performed with DU145 and TRAMP-C2 treated for 24 h with 100 μM FAC and RSL3 (0.3 μM for DU-145 cells and 1.0 μM for TRAMP-C2 cells). Representative images are reported. Data are the mean ± SEM of at least 3 experiments (*p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.0001).