Table 4.
Primers used in the study.
| Primer | Sequence (5′ to 3′) | Use |
|---|---|---|
| TK0548F | AAAAACATATGGCGCTGAGCGACAGGCTTGACC | Construction of expression plasmids |
| TK0548R | AAAGGATCCTTAAACGAGCTTTTTCTCCTTCAGG | |
| TK2268F | AAAAAAACATATGAGGTATAAGAAGAGAAAGTAC | |
| TK2268R | AAAGTCGACTCAGTGGTGGTGGTGGTGGTGCAGCTTCGAGAGGGCCT | |
| TK0548seqF1 | TCAGCGAGCTTATGCTCAAG | Sequencing |
| TK0548seqR1 | GCATTGGAAGGCCCGCGTTCGG | |
| TK2268seqF1 | CCCCAACGCAGGCATCC | |
| TK2268seqF2 | CCAAAATAGCCGAGGCAGGG | |
| TK2268seqF3 | TACATCCTCAGCGACGAGCCC | |
| TK2268seqF4 | CGGCAACGTTACGTCCTTCATCC | |
| TK2268seqF5 | TCGATATGACCAGCGAAGAC | |
| TK2268seqR1 | CCCTGCCTCGGCTATTTTGG | |
| dTK0548seqF1 | GGCAGCCCTGAGTGAGGGCGTTG | |
| dTK0548seqF2 | GGCGGTGTTGATGCGTTCTTC | |
| dTK0548seqF3 | ATTGAAATTCCGCAGAGCCATTG | |
| dTK0548seqF4 | CCGCGTCAACTTCGAGGCTCTC | |
| dTK0548seqF5 | GTGATACAATATGGAGAAGG | |
| dTK0548seqR1 | CATAATCCTCTCCCACGCTCCTCCGAAG | |
| dTK0548seqR2 | CCGGGGACATAACGAACTTC | |
| dTK0548seqR3 | CCTTCTCCATATTGTATCAC | |
| dTK0548seqR4 | CCTCACCACTCCCCAAAGTC | |
| TK0548seqF3 | ATGGTAATTAGCGATGAAGTTTACG | |
| TK0548seqF4 | CCTGTCCGGTCACCTTCGC | |
| TK0548seqF5 | TCAGCGAGCTTATGCTCAAG | |
| TK0548seqR2 | GAACGCCTGGTTGGCTCCGGTTAGG | |
| TK0548seqF6 | CCGAACGCGGGCCTTCCAATGC | |
| TK0548seqF2 | AAAGCCCGTTGAGGTTCC | |
| dTK2268seqF1 | GAATGACCTCACTCCTGTTC | |
| dTK2268seqF2 | CTGAACGATGAGACAGGTGATTG | |
| dTK2268seqF3 | CCCACCAGATAAGGGAAGCAATAAAAG | |
| dTK2268seqF4 | TTGTCGCAGAAAGACCCTTCGCCAAG | |
| dTK2268seqR1 | ATCCCCCCTCACATTTTTCC | |
| dTK2268seqR2 | AGTGCTATCGCGACGCTCCTC | |
| dTK2268seqR3 | TTCCAGCCAACCGCTTCGAC | |
| dTK2268seqR4 | CTCCCGCAGGGGCTAATAAACG | |
| dTK0548F1 | GAGAATTGAAACAAAAAGGTGGGTGGTG | Construction of disruption plasmids |
| dTK0548R1 | GAGATCAGACTGCGGGAGCGCTCTGCTTG | |
| dTK0548F2 | ATCAGGATAAGCTACGCCACGGCCTAC | |
| dTK0548R2 | TACCATCACCGCTCTCCCTTC | |
| dTK0548inF | CGCTGGGATGCAGGATGTTATC | |
| dTK0548inR | GTAGCCTTCTCCGGCTTTC | |
| dTK0548outF | ATTGAAACTATAGGGAAATATAGGG | |
| dTK0548outR | TAATCCCGAAGAAGGGCTACTC | |
| dTK2268F1 | ATCGTCGTCATCGAACTGAG | |
| dTK2268R1 | TGGAGACCTGGTCTTTTGTCC | |
| dTK2268F2 | ATGGAAAACCTGCTTGCCGTCTTCGTTTC | |
| dTK2268R2 | CGATGTTCCCCCCGGGCAGTTCGGGAATG | |
| dTK2268inF | ATGAGGTATAAGAAGAGAAAGTACTTCATGGCCGGCAGGATAA | |
| dTK2268inR | GGAACCACCTCACAGCTTCGAGAGGGCCTCTTTCATTC | |
| dTK2268outF | GGATCGCCGCCAGAACCCTTTC | |
| dTK2268outR | CATGGCATAGTTCCCTGCAAGCATTG |