FIGURE 11.

Summary of current and potential application 3PM. (A) Explored and potentially accessible regions in 3PM. Green shadows indicate areas that have been explored. Yellow shadows indicate potentially accessible areas. The white shadow denotes the regions beyond the present depth limitations and are inaccessible by surgery. (B) Fluorescent imaging application toolkit. Previous studies have explored the application of 3PM for various living components. A variety of fluorescence dyes and proteins have been analyzed. Several calcium indicators have been imaged through 3PM imaging, while various other types of sensors and probes through 3PM imaging have not been reported (indicated by gray).