Table 2.

Steps to be carried out to minimize the discrepancies in the estimation of Ki67 index

• Minimization of prefixation delay

• Division of surgical specimen to 5 to10-mm slices

• Fixation of specimen with neutral buffered formalin for 6 to 72 h

• In accordance with the type of specimen, fixation time should be altered

• Minimize the cold ischemia time

• Adopting “weighted global score” method recommended by IKWG instead of making use of “specific cell counting” or using “hot spot scoring”

• Standardize the cutoff for distinguishing low and high Ki-67 index