This work is licensed under a Figure 4.
MFSD4A-AS1 enhances lymphangiogenesis of vascular endothelial cells of PTC cells. (A and B) The effect of changed expression of MFSD4A-AS1 in PTC cells on human umbilical vein endothelial cells (HUVECs) in tube formation assay. Each bar represents the mean values ± s.d. of three independent experiments. *P < 0.05. (C and D) The effect of changed expression of MFSD4A-AS1 in PTC cells on migration ability of HUVECs in Transwell assay. Each bar represents the mean values ± s.d. of three independent experiments. *P < 0.05. (E) Real-time PCR analysis of the effect of MFSD4A-AS1 on the expression of multiple proliferation and migration of vascular endothelial cells-associated (angiogenesis-associated) genes. Pseudo-color scale values were log2 transformed. Transcript levels were normalized by GAPDH expression. The experiment was independently performed three times. (F and G) The effect of MFSD4A-AS1 on secretion level of VEGFA and VEGFC in PTC cells by enzyme-linked immunosorbent assay (ELISA). Each bar represents the mean values ± s.d. of three independent experiments. *P < 0.05.