TABLE 2.
T-CAST results for CCR5-targeting TALEN. Listed are OMTs with >20 hits and NBSs with >40 hits. TALEN combinations: LF, left TALEN arm in forward orientation; LR, left TALEN arm in reverse orientation; RF, right TALEN arm in forward orientation; RR, right TALEN in reverse orientation.
| Group | Chr. | Start | End | Reads | Hits | Combination | Closest gene | Annotation |
|---|---|---|---|---|---|---|---|---|
| ON | 3 | 46372560 | 46373160 | 3124912 | 12663 | LF.RR | CCR5 | 5′ UTR |
| OMT #1 | 4 | 153486313 | 153486513 | 46482 | 347 | LF.LR | TMEM131L | Intron |
| OMT #2 | 5 | 56330766 | 56330966 | 29865 | 211 | LF.LR | ANKRD55 | Distal Intergenic |
| OMT #3 | 1 | 169468608 | 169468808 | 7584 | 67 | LF.LR | SLC19A2 | Exon |
| OMT #4 | 3 | 110814452 | 110814652 | 10695 | 66 | LF.LR | NECTIN3 | Distal Intergenic |
| OMT #5 | 1 | 82062263 | 82062463 | 9351 | 58 | LF.LR | LOC101927434 | Distal Intergenic |
| OMT #6 | 2 | 207462882 | 207463082 | 8381 | 41 | LF.LR | MYOSLID-AS1 | Intron |
| OMT #7 | 17 | 1834349 | 1834549 | 9936 | 37 | RF.LR | RPA1 | Intron |
| OMT #8 | 2 | 224106645 | 224106845 | 7808 | 37 | LF.LR | SERPINE2 | Distal Intergenic |
| OMT #9 | 7 | 131476282 | 131476482 | 6576 | 33 | LF.LR | MKLN1 | Intron |
| OMT #10 | 2 | 156801241 | 156801441 | 4409 | 33 | LF.LR | GPD2 | Distal Intergenic |
| OMT #11 | 17 | 37438329 | 37438529 | 5278 | 30 | LF.LR | TBC1D3H | Intron |
| OMT #12 | 20 | 63138626 | 63138826 | 2568 | 24 | LF.LR | HAR1B | Distal Intergenic |
| NBS #1 | 14 | 94423347 | 94423547 | 19256 | 154 | RF.LR | SERPINA11 | Distal Intergenic |
| NBS #2 | 3 | 45844659 | 45844859 | 8582 | 41 | LF.LR | LZTFL1 | Intron |