Figure 10.

Changes in ROS levels and growth rates of C. reinhardtii cells by treatment of ascorbic acid (AA) following X-irradiation with 6 Gy. XR6 represents X-rays of 6 Gy. (A) The ROS level was calculated relative to the mock without AA treatment by the DCF signal at excitation 480 nm and emission 530 nm as described in the Materials and Methods section. (B) Cell density (OD₇₅₀) of C. reinhardtii 2 days after the second repetitive X-irradiation. Cells were incubated without or with 1 mM AA for 1 h at 25°C after X-irradiation, and were subjected to heterotrophic (H) cultivation in darkness or mixotrophic (M) cultivation under light for 2 days. Data represent the mean ± standard error (SE) with n = 6 from two independent experiments. Different letters indicate significant differences at p < 0.05 (one-way analysis of variance followed by Tukey’s honestly significant difference test). Digits in parentheses are percent differences in average between mock and irradiated cells.