Figure 8.

Synergistic growth stimulation of C. reinhardtii cells by cultivating in TAP medium containing sodium bicarbonate after repetitive X-irradiation with 6 Gy. BC05, BC10, and XR6 represent 5 and 10 mM NaHCO₃ and X-rays of 6 Gy, respectively. (A) A schematic diagram of repetitive X-irradiation and treatment of NaHCO₃. The absorbed dose of 6 Gy was repeatedly applied to the X-irradiated cells having an interval of 2 days. The cultivation of the mock was initiated at a cell density of 0.05 OD₇₅₀. The irradiated and/or treated samples were subjected to post-irradiation and/or post-treatment cultivation with the equivalent volume of the mock to cumulate a difference in the growth rate after each X-irradiation and/or treatment of NaHCO₃. (B) Cell density (OD₇₅₀) of C. reinhardtii 2 days after single or repetitive X-irradiation. Data represent the mean ± standard error (SE) with n = 6 from two independent experiments. Different letters indicate significant differences at p < 0.05 (one-way analysis of variance followed by Tukey’s honestly significant difference test).