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. 2023 Feb 20;14:1078205. doi: 10.3389/fphar.2023.1078205

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Copyright © 2023 Chen, Zhang, Wang, Tan, Lin, Qian, Li and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dapagliflozin ameliorates H/R-induced ROS and lipid peroxidation in H9C2 cells. (A–C) Representative fluorescence images of ROS and DHE staining (scale bar, 100 μM) and quantification of fluorescence intensity (n = 4, 5 per group). (D–F) The levels of lipid ROS were captured by using 2,7-dichlorofluorescein diacetate, oxidized lipids were captured by using C11 BODIPY and measured by flow cytometry in H9C2 cells pretreated with or without DAPA (n = 6 per group). (G) MDA content was assayed by a commercial kit (n = 4 per group). (H) The mRNA level of ACSL4 was measured by qPCR (n = 6 per group). (I) The protein level was measured by western blotting after treatment with 2.5 or 5 μM DAPA (n = 5 per group). Data are expressed as mean ± SD; One-way ANOVA followed by Tukey’s correction for post hoc multiple comparisons or Dunnett’s multiple comparison tests; #p < 0.05, ##p < 0.01 vs Control group; *p < 0.05, **p < 0.01 vs H/Rgroup.