FIG. 3.

The 87-kDa form of Gab1 is a functional protein involved in EGFR signaling in 10W−1 cells. (A and B) 10W−1L cells were serum starved for 20 h and left unstimulated or stimulated with 100 ng of EGF/ml for 1, 3, 5, and 15 min. Cells were lysed and subjected to immunoprecipitation (IP) of Gab1, followed by SDS–8% PAGE and anti-Gab1 (A) or anti-p-Tyr (B) blotting. (C) Gab1 (left) or SHP-2 (right) was immunoprecipitated (IP) from the lysates of 10W−1L cells left unstimulated or stimulated by 100 ng of EGF/ml for 1 min and analyzed by anti-SHP-2 (left) and anti-Gab1 (right) blottings, respectively.