FIG. 5.

Exclusive expression of Gab1 mRNA led by LSP sequence in 10W−1, especially in 10W−1L cells. One microgram of RNA from 10W+8, 10W−1E, and 10W−1L cells was subjected to reverse transcription reaction using oligo(dT)₁₈ primer. For some samples, reverse transcriptase (RT) was not included in the reaction (lanes 1, 3, 5, 7, 9, and 11). cDNA, in turn, was provided for PCR with either −14F or LSP as 5′ primer and 2104R as 3′ primer. PCR products were analyzed by 1.2% agarose gel and visualized with ethidium bromide staining and UV exposure.