Fig. 3.

Regulation of microglia phenotypes and inflammatory cytokines secretion in vitro. (A-B) Representative flow cytometry images (A) and immunostaining images (B) of CD206 (M2 marker) versus CD86 (M1 marker) fluorescence in primary microglia (Scale bar: 50 µm). (C-D) Quantification of the relative fluorescence intensity of CD206 versus CD86 in primary microglia tested by flow cytometry (C) and immunostaining (D). (E−J) Gene expression of pro-inflammatory and anti-inflammatory cytokines of BV2 cells determined by RT-qPCR. Data in (C-J) are expressed as mean ± SD (n = 6). **P < 0.05,**P < 0.01, ***P < 0.001, ****P < 0.0001.