Fig. 4.

In vivo targeting evaluation of Lipo@GB-DHA/DiD to ischemic brain in MCAO rats. (A) In vivo dynamic biofluorescence imaging of brains from MCAO rat models at 0.5, 1, 2, 4, 6, 12 and 24 h post-administration after 2 h of reperfusion (left) and ex vivo fluorescence images of the brains at 24 h post-administration (right). (B-C) Quantified fluorescence intensity by measuring the region of interest (ROI) in in vivo IVIS images and ex vivo images (n = 3). (D) Co-localization of Lipo@GB-DHA/DID with microglia in brains. (E) Representative immunostaining images of Lipo@GB-DHA with microglia/astrocytes in ischemic hemisphere by Image J (Scale bar: 20/200 μm). Data in (B, C) are expressed as mean ± SD (n = 3). **P < 0.01.