Fig. 7.

Study on remodeling of Lipo@GB-DHA in vivo. (A) Gating strategy of flow cytometry analysis for macrophages and microglia in brains. (B-D) Representative flow cytometry images (B) and quantifications (C-D) of CD11b⁺CD45^high macrophage and CD11b⁺CD45^med microglia in brains (n = 6). (E-F) Representative flow cytometry image (E) and quantifications (F) of M1/M2 cells in CD11b⁺CD45^med microglia in the ipsilateral side (n = 6). (G-H) Representative flow cytometry images (G) and quantification (H) of the proportion of CD3⁺ lymphocytes in the ipsilateral side (n = 6). (I) Immunostaining of CD206 and Iba-1 to determine the microglial phenotype in the ischemic penumbra treated with different formulations (Scale bar: 200 μm/20 μm). (J) Western blot analysis of autophagy-related proteins in brains. Data in (D-E, F-H) were expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ****P < 0.0001.