Figure 2.

Image of the western blot used to detect the YFP fusion proteins. The protein accumulation of (A) NbEDS1-YFP, (B) NbWRKY70-YFP, and (C) NbNDR1-YFP were detected by probing the blot with anti-HA-HRP antibodies. (A) tumefaciens strains carrying p35S::NbEDS1-YFP, NbWRKY70-YFP, and p35S::NbNDR1-YFP, were co-inoculated with pCas9 empty vector, pCas9-NbEDS1/NbNDR1 gRNA, and pCas9-NbWRKY70 gRNA onto the N. benthamiana leaf, respectively. CK: the protein was extracted from N. benthamiana leaf infiltrated with 10 mM MgCl₂ that served as a negative control. The blot membrane was stained with Ponceau S to confirm equivalent loadings. +: present of the indicated construct, -: absence of the indicated construct.