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. 2023 Feb 20;14:1111683. doi: 10.3389/fpls.2023.1111683

Figure 5.

Figure 5

Sanger sequencing chromatograms display the DNA sequence of GmKTI1 and GmKTI3 that is targeted for gene editing. The open reading frames of GmKTI1 and GmKTI3 in the pCas9-GmKTI1/GmKTI3 gRNA T0 transgenic soybean plants were amplified and sequenced. The double peaks in the sequencing chromatograms indicate the presence of both wild-type and mutant allele of sequences GmKTI1 (A) and GmKTI3 (B). The wild-type gene sequences are shown on the top of the sequencing chromatograms. The PAM sites are underlined.