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. 2023 Jan 4;24(3):e55762. doi: 10.15252/embr.202255762

Figure EV1. METTL3 but not WTAP protects METTL14 protein.

Figure EV1

  1. RT‐qPCR analysis of the expression levels of METTL3 and METTL14 in siRNA knockdown METTL3 cells. Data are mean ± SEM of three biological replicates and were analyzed by two‐tailed unpaired t‐test. ***P < 0.001; n.s., not significant.
  2. Immunoblots showing total METTL3 protein levels in FLAG‐tagged METTL3‐overexpressing cells. β‐actin was used as the loading control. The total METTL3/β‐actin densitometric ratio was recorded by ImageJ.
  3. Immunoblots showing METTL14 and WTAP protein levels in WTAP knockdown cells. β‐actin was used as the loading control. The METTL14 or WTAP/β‐actin densitometric ratio was recorded by ImageJ.
  4. Immunoblots showing METTL14 protein levels in FLAG‐tagged METTL3 or HA‐tagged WTAP‐overexpressing cells. β‐actin was used as the loading control. The METTL14/ β‐actin densitometric ratio was recorded by ImageJ.

Source data are available online for this figure.