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. 2023 Jan 4;24(3):e55762. doi: 10.15252/embr.202255762

Figure EV2. STUB1 interacts with METTL14 but is dispensable for regulating METTL14 mRNA levels.

Figure EV2

  • A
    List of the “Proteasome” category in the METTL14 interactome identified by MS, including five PSMAs, one PSMBs, four PSMCs, and eight PSMDs.
  • B
    Immunoblots showing PSMD3‐myc protein levels in myc‐tagged PSMD3‐ overexpressing cells. β‐actin was used as the loading control.
  • C
    RT‐qPCR analysis of the efficiency of shRNA‐knockdown for STUB1, UBR1, UBR5, and TRIM33. β‐actin was used as the reference. Data are mean ± SEM of three biological replicates and were analyzed by two‐tailed unpaired t‐test. (n.s., not significant; ***P < 0.001).
  • D, E
    Co‐IP of METTL14 and STUB1 in HEK293T and SK‐Cha‐1 cells.
  • F
    RT‐qPCR analysis of expression levels of STUB1 and METTL14 in STUB1 knockdown cells. Data are mean ± SEM of three biological replicates and were analyzed by two‐tailed unpaired t‐test. ***P < 0.001; n.s., not significant.

Source data are available online for this figure.